TABLE 1.
Bacterial strains used in this study to test the specificities of the narG and napA primersa
| Strain or isolate | Phylum | Nitrate reductase activity | Nitrate reductase enzymes | Q-PCR result for:
|
|
|---|---|---|---|---|---|
| narG | napA | ||||
| Bacillus senegalensis M1518 | Firmicutes | + | ND, ND | − | − |
| Bacillus cereus M944 ND | Firmicutes | + | Nar, ND | − | − |
| Bacillus thuringiensis | Firmicutes | + | Nar, ND | − | − |
| Bacillus megaterium | Firmicutes | + | Nar, ND | − | − |
| Streptomyces bluensis | Actinobacteria | + | ND, ND | − | − |
| Agrobacterium tumefaciens | Alphaproteobacteria | + | −, Nap | − | + |
| Bradyrhizobium japonicum 562 | Alphaproteobacteria | + | ND, ND | − | + |
| Bradyrhizobium japonicum USDA 110 | Alphaproteobacteria | + | −, Nap | − | + |
| Hyphomicrobium denitrificans DSM 1869 | Alphaproteobacteria | + | ND, ND | + | − |
| Sinorhizobium morelense SN611 | Alphaproteobacteria | + | ND, ND | − | + |
| Sinorhizobium meliloti 1021 | Alphaproteobacteria | + | −, Nap | − | + |
| Rhizobium meliloti 50 | Alphaproteobacteria | + | ND, ND | − | + |
| Paracoccus denitrificans Pd1222 | Alphaproteobacteria | + | Nar, Nap | + | + |
| Alcaligenes faecalis ATCC 8750 | Betaproteobacteria | + | ND, ND | − | − |
| Alcaligenes eutrophus H16 | Betaproteobacteria | + | Nar, Nap | + | + |
| Achromobacter cycloclastes ATCC 21921 | Betaproteobacteria | + | ND, ND | − | + |
| Escherichia coli JM109 | Gammaproteobacteria | + | Nar, Nap | + | + |
| Pseudomonas denitrificans CCUG 2519 | Gammaproteobacteria | + | ND, ND | + | − |
| Pseudomonas fluorescens C7R12 | Gammaproteobacteria | + | Nar, − | + | − |
a
Q-PCR, quantitative PCR; ND, not determined; −, not present.