TABLE 2.
Localization of iodate reductase in cell fractions of strain SCTa
Strain SCT cell fraction | Total U of iodate reductase (%) | Total U of catalase (%) |
---|---|---|
Crude extracts | 7.20 ± 0.94 (100) | NDb |
Periplasmic | 5.49 ± 0.29 (76.3) | 15.1 ± 11 |
Spheroplast | 1.61 ± 0.26 (22.4) | 1140 ± 49 |
Cytoplasmic | 1.26 ± 0.17 (17.5) | 1090 ± 57 |
Membrane | 0.129 ± 0.087 (1.8) | <10 |
Values are expressed as the means of total activity (U) ± standard deviations of the results obtained in at least three analyses. Iodate reductase activity was determined with MV as the electron donor. Values in parentheses represent percentages of the activity detected in each fraction.
ND, not determined.