TABLE 1.
Data summary of Q-PCR and PLFA groups for stimulated and control column sediment samples collected from sample ports 1 to 8
| Parameter | Value for sediment samples from:
|
Value for sediment samples from: | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stimulated column
|
Control column
|
|||||||||||||||||||
| EtOH 1 | EtOH 2 | EtOH 3 | EtOH 4 | EtOH 5 | EtOH 6 | EtOH 7 | EtOH 8 | Avg | SD | C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | Avg | SD | |
| PLFA | ||||||||||||||||||||
| Viable biomass (cells/g sediment) | 4.28E+08 | 6.46E+08 | 4.80E+08 | 6.49E+08 | 6.99E+08 | 5.18E+08 | 8.16E+08 | 4.53E+08 | 5.86E+08 | 1.28E+08 | 4.36E+06 | 4.39E+06 | 8.98E+06 | 1.48E+07 | 6.59E+06 | 4.43E+06 | 1.29E+07 | 2.08E+06 | 7.32E+06 | 4.24E+06 |
| Community structure (% total PLFA) | ||||||||||||||||||||
| Terminally branched saturates | 14.6 | 16.0 | 20.0 | 22.2 | 17.3 | 21.4 | 20.4 | 20.8 | 19.1 | 2.57 | 17.6 | 16.4 | 14.5 | 14.0 | 15.2 | 14.3 | 10.3 | 10.4 | 14.1 | 2.43 |
| Branched monounsaturates | 3.16 | 3.83 | 3.94 | 5.30 | 3.76 | 3.86 | 4.29 | 3.88 | 4.00 | 0.57 | 3.83 | 2.28 | 3.28 | 2.92 | 2.52 | 2.76 | 1.83 | 1.60 | 2.63 | 0.69 |
| Polyunsaturates | 0.242 | 0.230 | 0.448 | 0.357 | 0.423 | 0.434 | 0.510 | 0.788 | 0.43 | 0.16 | 1.75 | 1.05 | 1.67 | 1.59 | 1.54 | 1.39 | 1.25 | 0.72 | 1.37 | 0.33 |
| Midchain-branched saturates | 3.15 | 5.15 | 4.69 | 4.82 | 6.80 | 7.56 | 8.92 | 8.95 | 6.26 | 1.99 | 9.52 | 8.65 | 9.15 | 9.11 | 11.7 | 12.3 | 10.5 | 9.49 | 10.0 | 1.23 |
| Normal saturates | 16.6 | 21.3 | 18.9 | 18.8 | 19.9 | 20.3 | 20.6 | 20.6 | 19.6 | 1.39 | 22.2 | 26.0 | 22.8 | 23.7 | 23.9 | 23.8 | 27.1 | 28.5 | 24.7 | 2.07 |
| Monounsaturates | 61.9 | 53.4 | 52.0 | 48.4 | 51.8 | 46.3 | 45.1 | 44.9 | 50.5 | 5.31 | 45.1 | 45.7 | 48.6 | 48.7 | 45.2 | 45.4 | 49.0 | 49.3 | 47.1 | 1.81 |
| Hydroxy | 0.248 | 0.062 | 0.054 | 0.116 | 0.111 | 0.130 | 0.129 | 0.150 | 0.13 | 0.06 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Shannon-Weiner diversity index | 2.59 | 2.82 | 2.88 | 2.88 | 2.93 | 3.04 | 3.04 | 3.14 | 2.91 | 0.16 | 3.09 | 2.88 | 3.03 | 2.97 | 3.04 | 3.09 | 2.79 | 2.65 | 2.94 | 0.15 |
| Metabolic status (ratio) | ||||||||||||||||||||
| cy17:0/16:1w7c | 0.084 | 0.051 | 0.060 | 0.058 | 0.053 | 0.074 | 0.047 | 0.083 | 0.064 | 0.014 | 0.377 | 0.329 | 0.549 | 0.394 | 0.488 | 0.411 | 0.496 | 0.676 | 0.465 | 0.104 |
| cy19:0/18:17c | 0.057 | 0.080 | 0.094 | 0.103 | 0.113 | 0.096 | 0.181 | 0.129 | 0.107 | 0.035 | 0.260 | 0.267 | 0.293 | 0.231 | 0.256 | 0.262 | 0.224 | 0.213 | 0.251 | 0.024 |
| Total cyc/monoa | 0.141 | 0.131 | 0.154 | 0.161 | 0.166 | 0.169 | 0.228 | 0.212 | 0.170 | 0.031 | 0.637 | 0.596 | 0.842 | 0.625 | 0.744 | 0.673 | 0.720 | 0.890 | 0.716 | 0.098 |
| 16:1w7t/16:1w7c | 0.070 | 0.070 | 0.094 | 0.114 | 0.099 | 0.122 | 0.102 | 0.155 | 0.103 | 0.026 | 0.063 | 0.054 | 0.080 | 0.065 | 0.062 | 0.069 | 0.054 | 0.063 | 0.064 | 0.008 |
| 18:1w7t/18:1w7c | 0.034 | 0.036 | 0.033 | 0.033 | 0.033 | 0.035 | 0.064 | 0.075 | 0.043 | 0.016 | 0.054 | 0.038 | 0.076 | 0.063 | 0.062 | 0.066 | 0.036 | 0.072 | 0.058 | 0.014 |
| Total trans/cisb | 0.104 | 0.106 | 0.127 | 0.146 | 0.131 | 0.157 | 0.165 | 0.230 | 0.146 | 0.038 | 0.117 | 0.092 | 0.156 | 0.127 | 0.124 | 0.135 | 0.090 | 0.134 | 0.122 | 0.021 |
| i15:0/a15:0 | 0.831 | 0.767 | 0.725 | 0.453 | 0.617 | 0.540 | 0.670 | 0.838 | 0.680 | 0.129 | 1.21 | 1.37 | 1.28 | 1.47 | 1.39 | 1.38 | 1.45 | 1.38 | 1.368 | 0.081 |
| i17:0/a17:0 | 0.245 | 0.198 | 0.193 | 0.181 | 0.172 | 0.229 | 0.249 | 0.295 | 0.220 | 0.039 | 0.695 | 0.859 | 0.648 | 0.736 | 0.702 | 0.713 | 0.720 | 0.945 | 0.752 | 0.092 |
| Total iso/anteisoc | 1.08 | 0.965 | 0.917 | 0.635 | 0.789 | 0.769 | 0.919 | 1.13 | 0.900 | 0.154 | 1.91 | 2.23 | 1.92 | 2.21 | 2.09 | 2.10 | 2.17 | 2.33 | 2.120 | 0.138 |
| DMAs (pmol/g sediment) | 206 | 343 | 397 | 326 | 479 | 531 | 1,012 | 612 | 488 | 231 | 16.5 | 1.79 | 28.2 | 18.3 | 15.9 | 8.65 | 14.9 | 8.19 | ||
| UQ/MQ ratio | 2.53 | 2.14 | 2.36 | 4.07 | 4.33 | 3.68 | 3.19 | 0.87 | 3.49 | 2.77 | 2.97 | 2.45 | 2.12 | 1.68 | 1.38 | 0.390 | 2.16 | 0.926 | ||
| Q-PCR (copies/g sediment)d | ||||||||||||||||||||
| Eubacterial 16S rRNA | 1.23E+09 | 3.94E+08 | 2.08E+09 | 1.88E+09 | 3.11E+09 | 2.00E+09 | 2.65E+09 | 2.07E+09 | 1.93E+09 | 7.76E+08 | 5.00E+07 | 5.83E+07 | 5.83E+07 | 6.91E+06 | 2.11E+07 | 1.59E+07 | 8.22E+06 | 3.68E+06 | 3.08E+07 | 2.71E+07 |
| nirS | 2.89E+08 | 3.08E+07 | 3.06E+08 | 3.55E+08 | 4.35E+08 | 4.53E+08 | 5.99E+08 | 1.17E+09 | 4.55E+08 | 3.10E+08 | 1.02E+07 | 8.20E+06 | 7.69E+06 | 1.17E+06 | 2.80E+06 | 2.44E+06 | 9.57E+06 | 1.65E+06 | 5.46E+06 | 3.55E+06 |
| nirK | 9.31E+07 | 5.56E+07 | 1.85E+08 | 2.07E+08 | 2.20E+08 | 2.46E+08 | 2.45E+08 | 3.97E+08 | 2.06E+08 | 9.72E+07 | 4.48E+06 | 9.99E+06 | 1.80E+07 | 5.12E+06 | 9.35E+06 | 4.43E+06 | 2.11E+04 | ND | 6.42E+06 | 5.55E+06 |
| Deltaproteobacteria | 3.88E+05 | ND | 4.67E+07 | 3.46E+08 | 1.78E+08 | 2.22E+08 | 1.49E+08 | 3.52E+08 | 1.62E+08 | 1.32E+08 | 3.08E+07 | 3.25E+06 | 4.05E+06 | 2.44E+04 | 5.42E+05 | 5.42E+05 | 2.44E+04 | 2.36E+03 | 4.91E+06 | 9.90E+06 |
| Geobacteraceae | 108* | ND | 205* | 642* | 4.64E+04 | 1.88E+04 | 4.82E+03 | 1.98E+04 | 1.13E+04 | 1.54E+04 | <100 | <100 | <100 | ND | ND | <100 | 112* | ND | ||
| Methanogens | ND | ND | ND | ND | ND | ND | ND | ND | 6.61E+05 | 5.56E+06 | 2.25E+05 | 4.18E+05 | 2.64E+05 | 3.27E+05 | 2.64E+05 | 3.22E+05 | 1.01E+06 | 1.73E+06 | ||
| Methylotrophs | 6.24E+06 | ND | 5.54E+05 | 1.60E+05 | 1.35E+05 | 1.31E+05 | 1.72E+05 | 4.34E+05 | 9.78E+05 | 2.00E+06 | 3.72E+05 | 2.15E+07 | 5.53E+05 | 2.82E+05 | 1.90E+05 | 5.14E+05 | 3.63E+05 | ND | 2.97E+06 | 7.00E+06 |
a
cyc/mono, ratio of cyclopropane to monoenoic precursor fatty acids.
b
trans/cis, ratio of monounsaturated trans to cis isomers.
c
iso/anteiso, ratio of iso- to anteiso-saturated fatty acids.
d
*, estimated value; <100, below detection limit; ND, not detected (sample not processed).