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Western blotting of L. lactis cellular fractions revealed with anti-YjgB antibodies. TIL903, control strain; TIL904, strain overexpressing yjgB under the control of a nisin-inducible promoter. Nisin was added at a concentration of 2.5 ng ml−1 and an OD600 of 0.4 and growth was continued for 4 h before cells were harvested for fractionation. SN, culture supernatant; CW, cell wall fraction; PR, protoplast fraction.
