TABLE 2.
Substrate specificity of purified YjgB-His with respect to purified reduced muropeptides
| Peaka | Muropeptideb | % Digested muropeptidec |
|---|---|---|
| 1 | DS-Tri | 5.4 |
| 2 | DS-Tetra | 70.5 |
| 3 | DS-Tri-D | 2.1 |
| 4 | DS-Tri*-N | 1.7 |
| 5 | DS-Tri-N | 3.3 |
| 6 | DS-Tetra-D | 84.2 |
| 7 | DS-Penta-D | 98.3 |
| 8 | DS-Tetra-N | 92.2 |
| 9 | DS-Penta-N | 92.5 |
a
Peak numbers refer to chromatogram shown in Fig. 3.
b
DS-Tri, disaccharide tripeptide (l-Ala-γ-D-Gln-l-Lys); DS-Tri*, disaccharide tripeptide (l-Ala-γ-d-Glu-l-Lys); DS-Tetra, disaccharide tetrapeptide (l-Ala-γ-d-Gln-l-Lys-d-Ala); DS-Penta, disaccharide pentapeptide disaccharide (l-Ala-γ-d-Gln-l-Lys-d-Ala-d-Ala); disaccharide, GlcNAc-MurNAc (GlcNAc, N-acetylglucosamine, MurNAc, N-acetylmuramic acid); D, d-Asp; N, d-Asn.
c
The percentage of digested muropeptide was calculated as the ratio of the DS-di peak area to the sum of the peak areas of the nondigested muropeptide substrate and the DS-di product.