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ATR-FTIR spectra of P. fluorescens planktonic bacteria harvested at the end of the exponential phase (spectrum a) and a 6-h-old P. fluorescens biofilm formed by successive flow of a bacterial suspension for 3 h and sterile LB medium (0.5 g/liter) over the germanium IRE (spectrum b). The spectra have been vertically shifted for clarity and normalized to the amide II band. str., stretch; sym., symmetric; asym., asymmetric; a.u., arbitrary units.
