TABLE 1.
Real-time PCR primer and probe sequences utilized in the multiplex assay
| Primer or probe | Sequence (5′ to 3′) | Modificationsa |
|---|---|---|
| tdh forward | TCCCTTTTCCTGCCCCC | |
| tdh reverse | CGCTGCCATTGTATAGTCTTTATC | |
| tdh probe | TGACATCCTACATGACTGTG | 5′ FAM to 3′ MGBNFQ |
| trh forward | TTGCTTTCAGTTTGCTATTGGCT | |
| trh reverse | TGTTTACCGTCATATAGGCGCTT | |
| trh probe | AGAAATACAACAATCAAAACTGA | 5′ TET to 3′ MGBNFQ |
| tlh forward | ACTCAACACAAGAAGAGATCGACAA | |
| tlh reverse | GATGAGCGGTTGATGTCCAA | |
| tlh probe | CGCTCGCGTTCACGAAACCGT | 5′ TxRED to 3′ BHQ2 |
| IAC forward | GACATCGATATGGGTGCCG | |
| IAC reverse | CGAGACGATGCAGCCATTC | |
| IAC probe | TCTCATGCGTCTCCCTGGTGAATGTG | 5′ Cy5 to 3′ BHQ2 |
a
FAM, 6-carboxyfluorescein; MGBNFQ, minor groove binding nonfluorescent quencher; TET, tetrachlorofluorescein; TxRED, Texas Red; BHQ2, black hole quencher 2.