TABLE 2.
Strains tested for assay specificity
| Species (no. of isolates) | Gene target
|
||
|---|---|---|---|
| tlh | tdh | trh | |
| V. parahaemolyticus (5) | + | − | − |
| V. parahaemolyticus (38) | + | + | − |
| V. parahaemolyticus (13) | + | − | + |
| V. parahaemolyticus (61) | + | + | + |
| V. cholerae (8) | − | − | − |
| V. mimicus (6) | − | − | − |
| V. fluvialis (6) | − | − | − |
| V. hollisae (1) | − | − | − |
| V. hollisae (3)a | − | + | − |
| V. metchnikovii (1) | − | − | − |
| V. vulnificus (10) | − | − | − |
| V. alginolyticus (1) | − | − | − |
a
CT values for tdh were 33.28, 35.22, and 36.23; V. parahaemolyticus strains generated CT values of 15.54 to 18.72 for the tdh gene at similar concentrations.