TABLE 4.
Alaskan oyster samples positive for tlh, tdh, and/or trh by real-time multiplex PCR and culture
| Sampleb | MPN value (MPN/g) for target gene ina:
|
|||||
|---|---|---|---|---|---|---|
| Real-time PCR
|
Culture
|
|||||
| tlh | tdh | trh | tlh | tdh | trh | |
| 872 | 40.5 | 40.5 | 40.5 | 4.05 | ND | 4.05 |
| 873 | 40.5 | 40.5 | 40.5 | 0.405 | ND | 0.405 |
| 888 | 4.05 | 4.05 | 405 | 4.05 | 4.05 | 4.05 |
| 889 | 10.99 | 10.99 | 10.99 | 4.05 | 4.05 | 4.05 |
| 936 | 4.05 | 4.05 | 4.05 | 1.099 | 1.099 | 1.099 |
| 937 | 4.05 | 4.05 | 4.05 | 0.405 | 0.405 | 0.405 |
| 1022 | 0.1099 | 0.0405 | 0.405 | 0.1099 | ND | ND |
| 1023 | 0.0405 | 0.0405 | 0.0405 | 0.0405 | 0.0405 | 0.0405 |
| 1047 | 0.405 | 0.405 | 0.405 | 0.405 | ND | ND |
| 1048 | 0.1099 | 0.0405 | 0.0405 | ND | ND | ND |
| 1054 | >0.1099 | >0.1099 | >0.1099 | ND | ND | ND |
| 1072 | ND | ND | >1.099 | ND | ND | ND |
| 1073 | ND | ND | 10.99 | ND | ND | ND |
| 1229 | 0.0405 | 0.0405 | 0.0405 | ND | ND | ND |
a
ND, not detected; limit of detection, <0.0405. MPN/g, MPN value per gram of oyster.
b
Samples that were negative for all gene targets by both methods are omitted from the table.