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. 2007 Aug 3;73(18):5911–5918. doi: 10.1128/AEM.00952-07

FIG. 2.

FIG. 2.

Transfer of R995 + SPI-2 to different bacterial hosts as shown via PCR analysis. Plasmid DNA isolated from the indicated transconjugant strains containing either R995 or R995 + SPI-2 was used as a template in PCR analysis with primers hybridizing to portions of the indicated SPI-2 genes. The PCR products were run on DNA agarose gels and stained with ethidium bromide. Odd-numbered and even-numbered lanes indicate samples from strains containing R995 or R995 + SPI-2, respectively. The lane marked “+” indicates PCRs where chromosomal DNA from wild-type S. enterica serovar Typhimurium strain χ3339 was used as a template. Abbreviations: S.t., S. enterica serovar Typhimurium; E.c., E. coli; P.a., P. aeruginosa.