TABLE 2.
RT and PCR primers
| Primer | Sequence (5′-3′) | Annealing temp (°C) | Approx product size (bp) | Genome(s) based ona |
|---|---|---|---|---|
| Mpl.sc1F | TIGCIGGWGAYTWYARM | 50 | 500 | JP-A, JP-B, RsRNAV |
| Mpl.sc1R | YTCCTTWTCRGSCATKGTA | |||
| Mpl.sc2F | ITWGCIGGIGATTWCA | 43 | 500 | HaRNAV, JP-A, RsRNAV |
| Mpl.sc2R | CKYTTCARRAAWTCAGCATC | |||
| Mpl.sc3F | TIATIGMKGGIGAYTA | 49 | 500 | HaRNAV, JP-A, SssRNAV |
| Mpl.sc3R | TTMARGAAIKMAGCATCTT | |||
| Mpl.cdhF | GMIGGTGAYTAYAGCGCTTWYGAY | 44 | 500 | JP-A, JP-B, RsRNAV, HaRNAV, SssRNAV |
| Mpl.cdhR | ATACCCAATGCCTYTTIARRAA |
a
The primers were designed from an alignment of the genomes listed.