TABLE 1.
Medium | MCYST cell quota (fg cell−1), only intracellularb | MCYST production (fg cell−1 day−1), intracellular + extracellularc | MCYST-LR/[d-Asp3]-MCYST-LR ratiob | % of MCYST extracellularb | Chl a cell quota (fg cell−1)b | Phycocyanin cell quota (pg cell−1)b | Fv,rel (10−4 cell−1)d | Cell diam (μm)b |
---|---|---|---|---|---|---|---|---|
Na (high Ci,i) | 18.0 (0.95) | 0.65 (0.3) | 0.25 (0.01) | 0.21 (0.01) | 25.7 (3.5) | 1.20 (0.02) | 2.55 (0.14) | 2.35 (0.01) |
K (low Ci,i) | 23.7 (0.96) | 2.2 (0.3) | 0.31 (0.03) | 0.47 (0.03) | 29.2 (1.6) | 2.46 (0.16) | 2.64 (0.15) | 2.45 (0.04) |
P value | 0.01e | 0.0001e | 0.08 | 0.0007e | 0.15 | 0.001e | 0.049e | 0.065 |
See Materials and Methods for an explanation of the treatments. All values are averages for the exponential growth phase. The values in parentheses are standard errors.
Means for exponential growth were tested.
A linear mixed model (38) with MCYST as the dependent variable and medium as the independent variable was used; the intercept was assumed to be constant, and only the slope for each replicate with time as a random effect was tested.
Fv,rel, relative units of variable fluorescence. Data were fitted by using Fv,rel = a · e−b · t and parameter b was tested.
Significant at α = 0.05.