TABLE 1.
Growth and harvesting conditions, suspending liquid, and suspension density for the microbial strains used in this study
| Microorganism(s) | Growth medium | Centrifugation | Buffer | Suspension density (cells/ml) |
|---|---|---|---|---|
| S. oralis J22 | Todd-Hewitt broth (Oxoid, Basingstoke, United Kingdom) | 10,000 × g, three times for 5 min, 10°C | Adhesion buffer (3.73 g/liter KCl, 0.174 g/liter K2HPO4, 0.136 g/liter KH2PO4, 0.147 g/liter CaCl2·2H2O; pH 6.8) | 3 × 108 |
| P. aeruginosa SG81 | Pseudomonas isolation broth (20 g/liter Bacto peptone, 10 g/liter K2SO4, 1.4 g/liter MgCl2·6H2O, 0.025 g/liter Triclosan, 25.2 g/liter glycerol; pH 7.0) | 10,000 × g, three times for 5 min, 10°C | 0.14 M NaCl | 2 × 108 |
| E. faecalis BS385 and BS1037 | TSB (Oxoid, Basingstoke, United Kingdom) | 6,500 × g, three times for 5 min, 10°C | 10 mM potassium phosphate buffer (0.87 g/liter K2HPO4, 0.68 g/liter KH2PO4) | 3 × 108 |
| C. albicans SC5314, MB02, and MB10 | TSB (Oxoid, Basingstoke, United Kingdom); for biofilm growth, yeast nitrogen base (Difco) without amino acids (Becton Dickinson, Sparks, MD) | 5,000 × g, once for 10 min, 10°C | Phosphate-buffered saline (8.76 g/liter NaCl, 0.87 g/liter K2HPO4, 0.68 g/liter KH2PO4; pH 7.0) | 1 × 107 |