TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Bacterial strains | ||
| Escherichia coli DH5α | DH1 F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 | N. T. Keen |
| Xylella fastidiosa | Wild-type X. fastidiosa A05 | 10 |
| X. fastidiosa algU::nptII | Tn5 insertional mutation in algU homologue of X. fastidiosa A05 | This work |
| Plasmids | ||
| pGEM-T Easy | Apr; cloning vector | Promega |
| pUC129 | Apr; cloning vector | New England BioLabs |
| pUC1284 | Apr; 0.891-kb fragment including algU ORF cloned into pUC129 | This work |
| pUC12841 | Apr Kmr; Tn5 insertion within algU ORF in pUC1284 | This work |