FIG. 1.

(A) Schematic picture of the processed gene fusion products anchored to the cell wall in strains Sc:Z_wt, Sc:Z_N28A, and Sc:Z_K35A (20). Z* represents the three different single-amino-acid mutants, Z_wt, Z_N28A, and Z_K35A. PP, propeptide from Staphylococcus hyicus lipase; Z, engineered IgG binding domain derived from staphylococcal protein A; ABP, albumin binding protein derived from streptococcal protein G and used as a normalization tag (20); X, charged repetitive region derived from staphylococcal protein A, postulated to interact with the peptidoglycan cell wall; M′, the processed and covalently cell wall-anchored form of the M sequence of staphylococcal protein A. (B) Schematic picture of the processed gene fusion products anchored to the cell wall in the new strain Sc:ABD_wt. ABD_wt, albumin binding domain derived from streptococcal protein G (44); Z_wt, engineered IgG binding domain derived from staphylococcal protein A and used as a dimer for normalization, according to the previously described principle (20).