Color image of Dragon Spring. Almost all sampling occurred in the yellow zone, where the solid-phase material is composed of S0. The main transect points are numbered 1 to 5. Sampling for some experiments occurred at many locations not always corresponding exactly to the primary transect points. The water temperatures shown were measured on 15 June 2003, were the typical upper boundary temperatures observed at each transect point during this study, and illustrate the temperature gradient in this portion of the outflow channel. Vertical arrows indicate the sampling sites for DNA used for PCR-DGGE analysis and correspond to lanes A, B, and C in Fig. 7. This color image is modified (with permission of the publisher) from a photograph taken by T.R.M. and used previously (36).