FIG. 3.
DegU and ComA control the expression of bacillomycin D. (A and B) Strains AK9 and AK10, carrying the two bmyD::lacZ fusions with the longest upstream regions (see also Fig. 2A), and their degU (A) and comA (B) mutant derivatives were grown in Difco medium at 37°C, and optical densities (OD) (closed symbols) and β-galactosidase activities (open symbols) were determined during growth. Squares, AK9; diamonds, AK10; triangles, mutant derivatives of AK9 (AK32 in panel A and AK22 in panel B); circles, mutant derivatives of AK10 (AK33 in panel A and AK23 in panel B). (C) Effects of degU and comA mutations on the activity of the bmy operon promoter (Pbmy). The intensities of the obtained transcripts indicate the effects exerted by the respective mutations on the activity of the Pbmy promoter. 1, FZB42; 2, TF1 (degU); 3, CH23 (comA). +1 and the adjacent arrow indicate the transcriptional start.