Production of bacillomycin D by FZB42 derivatives as indicated by growth inhibition of the Saccharomyces cerevisiae indicator strain used in the agar diffusion assay. (A) FZB42, CH4 (yczE), and TF1 (degU). (B) Top, CH41 (PbmyD::Pspac), CH42 (PbmyD::Pspac yczE), and CH43 (PbmyD::Pspac degU) without IPTG; bottom, CH41 (PbmyD::Pspac), CH42 (PbmyD::Pspac yczE), and CH43 (PbmyD::Pspac degU) after induction by IPTG.