TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| Rhodococcus sp. | ||
| RHA1 | Wild type | 17 |
| RHA027 | ΔprmA RHA1 with prmA deletion | This study |
| RHA028 | ΔalkB RHA1 with alkB deletion | This study |
| E. coli | ||
| DH5α | Host used for cloning the mutagenic plasmid | Bethesda Research |
| S17-1 | Donor strain for conjugation | 27 |
| TG1/pBS(Kan)PrMO.RHA1 | E. coli host containing gene cluster for PrMO | This study |
| TG1/pBS(Kan)AlkMO.RHA1 | E. coli host containing gene cluster for AlkMO | This study |
| Plasmids | ||
| pK18mobsacB | 5.7-kb mobilizable suicide vector used for triple ligation; sacB alphII | 30 |
| pΔprmA | 2.2-kb fusion PCR fragment flanking ΔprmA cloned into pK18mobsacB; used to make strain RHA027 | This study |
| pΔalkB | 2.2-kb fusion PCR fragment flanking ΔalkB cloned into pK18mobsacB; used to make strain RHA028 | This study |