TABLE 3.
Transcriptional responses of selected ORFs to β-glycoside linkage (cellobiose) independent of S0 in P. furiosus
| Function | GeneID no. | Transcriptional response (fold change)a
|
Annotation | ||
|---|---|---|---|---|---|
| Cellobiose vs maltose | Cellobiose + S0 vs maltose + S0 | Cellobiose + S0 vs tryptone + S0 | |||
| Cellobiose hydrolysis | PF0073 | 35.5 | 22.7 | 8.0 | β-Glucosidase |
| PF0074 | 7.9 | 8.4 | 4.8 | Alcohol dehydrogenase, short chain | |
| PF0075 | 14.5 | 12.5 | 6.3 | Alcohol dehydrogenase, class IV | |
| PF0076 | 2.6 | 2.2 | 2.2 | β-1,3-Endoglucanase | |
| Chitin transport and utilization | PF0355 | NC | NC | NC | VapC1 |
| PF0355.1 | NA | NA | NA | VapB1 | |
| PF0356 | NC | NC | NC | GH1, β-glycosidase | |
| PF0357 | NC | NC | NC | ABC transporter, binding protein | |
| PF0358 | NC | NC | NC | ABC transporter, permease | |
| PF0359 | NC | NC | NC | ABC transporter, permease | |
| PF0360 | 7.7 | 11.3 | 6.2 | ABC transporter, ATP-binding protein | |
| PF0361 | 4.6 | 7.9 | 4.9 | ABC transporter, ATP-binding protein | |
| PF0362 | NC | NC | NC | Glucosamine-fructose-6-phosphate aminotransferase | |
| PF0363 | NC | NC | NC | Exo-β-d-glucosaminidase | |
| Transport | PF0429 | 2.9 | 3.9 | 6.1 | NA+/proline symporter |
| Carbohydrate utilization | PF0496 | 3.2 | 2.2 | 2.8 | Transcriptional regulator, TrmB homolog |
| Gluconeogenesis | PF0613 | 2.9 | 2.4 | 3.1↓ | Archaeal fructose 1,6-bisphosphatase |
| Polysaccharide synthesis | PF0765 | 5.9 | 18.4 | 6.0 | UDP-N-acetyl-d-mannosaminuronate dehydrogenase |
| PF0766 | 3.3 | 8.1 | 4.4 | Dehydrogenase | |
| PF0767 | 4.8 | 13.5 | 5.3 | Aspartate aminotransferase | |
| PF0768 | 4.6 | 11.9 | 4.5 | Acetyltransferase | |
| Regulation/polysaccharide anabolism | PF0776 | 2.2 | 3.8 | 4.6 | Nucleic acid-binding protein, PIN domain |
| PF0777 | 4.3 | 8.8 | 7.9 | Capsular polysaccharide biosynthesis | |
| β-Glucan uptake | PF1206 | 2.4 | 1.6 | 1.6 | VapC12 toxin |
| PF1207 | 5.2 | 5.0 | 3.9 | VapB12 antitoxin | |
| PF1208 | 12.5 | 8.5 | 5.5 | β-Mannosidase | |
| PF1209 | NC | NC | NC | ABC transporter, binding protein | |
| PF1210 | NC | NC | NC | ABC transporter, permease | |
| PF1211 | 2.4 | 3.1↓ | 3.0 | ABC transporter, permease | |
| PF1212 | NC | NC | NC | ABC transporter, uptake protein | |
| PF1213 | NC | NC | NC | ABC transporter, binding protein | |
| Cofactor biosynthesis | PF1674 | 7.4 | 12.0 | 5.3 | Protein-tyrosine phosphatase |
| PF1675 | NC | NC | 2.0↓ | Aspartate racemase | |
| PF1676 | 9.6 | 19.4 | 8.3 | Biotin-(acetyl-CoA carboxylase) ligase | |
| Sugar transport | PF1695 | NC | NC | NC | ABC transporter, binding protein |
| PF1696 | 15.2 | 34.1 | 10.7 | ABC transporter, ATPase | |
| PF1697 | 9.4 | 45.3 | 7.7 | ABC transporter, permease | |
| PF1698 | NC | NC | NC | ABC transporter, permease | |
a
NC, no change (≤1.5-fold change); NA, not available; ↓, down-regulated.