TABLE 5.
Transcriptional responses of selected ORFs significantly regulated by tryptone independent of glycoside type in P. furiosus
| Function | GeneID no. | Transcriptional response (fold change)a
|
Annotationb | |
|---|---|---|---|---|
| Tryptose + S0 vs cellobiose + S0 | Tryptone + S0 vs maltose + S0 | |||
| Gluconeogenesis | PF0613 | 3.1 | 7.3 | Fructose-1,6-bisphosphatase |
| PF0289 | 2.6 | 3.1 | Phosphoenolpyruvate carboxykinase | |
| PF1874 | 4.4 | 5.7 | Glyceraldehyde-3-phosphate dehydrogenase | |
| Transcriptional regulation | PF0054 | 2.6 | 2.2 | Regulator, Lrp/AsnC family |
| PF0055 | 3.5 | 2.2 | Putative HTH transcription regulator | |
| PF0056 | 2.1 | 2.2 | Putative carbohydrate binding protein | |
| Aminotransferase | PF0121 | 3.1 | 2.6 | Aromatic amino acid aminotransferase |
| PF1497 | 3.4 | 2.2 | Alanine aminotransferase | |
| Oligopeptide ABC transporter | PF0191 | 5.4 | 11.2 | Oligopeptide transporter, permease |
| PF0192 | 4.7 | 9.6 | Oligopeptide transporter, permease | |
| PF0193 | 4.5 | 10.6 | Oligopeptide transporter, ATPase | |
| PF0194 | 3.6 | 8.1 | Oligopeptide transporter, ATPase | |
| PF0195 | 5.6 | 8.6 | Hypothetical protein | |
| Acyl-CoA synthetase II | PF0233 | 2.8 | 2.3 | Acyl-CoA synthetase II homolog |
| PF1838 | 3.0 | 2.4 | Acyl-CoA synthetase II homolog | |
| PF0532 | 2.2 | 2.4 | Acyl-CoA synthetase II | |
| Acetyl-CoA synthase | PF0972 | 3.5 | 7.5 | Acyl carrier protein synthase |
| PF0973 | 3.2 | 6.3 | Acetyl-CoA synthase | |
| PF0974 | 3.3 | 7.8 | Hypothetical protein | |
| 2-Keto acid ferredoxin oxidoreductase | PF0533 | 2.0 | 1.6 | IOR, alpha subunit |
| PF0534 | 2.1 | 2.0 | IOR, beta subunit | |
| PF1767 | 15.0 | 10.8 | KGOR, delta subunit | |
| PF1768 | 14.2 | 10.5 | KGOR, gamma subunit | |
| PF1769 | 15.2 | 11.0 | KGOR, beta subunit | |
| PF1770 | 12.7 | 9.6 | KGOR, alpha subunit | |
| PF1771 | 12.9 | 8.9 | KOR, alpha subunit | |
| PF1772 | 9.1 | 7.6 | KOR, beta subunit | |
| PF1773 | 9.3 | 7.9 | KOR, gamma subunit | |
| Glycolysis | PF0215 | 2.1↓ | 3.0↓ | Enolase |
| PF0312 | 2.3↓ | 2.4↓ | ADP-dependent glucokinase | |
| PF1784 | 2.3↓ | 2.7↓ | ADP-dependent phosphofructokinase | |
| PF1956 | 2.1↓ | 2.6↓ | Fructose-1,6-bisphosphate aldolase | |
| PF1959 | 2.2↓ | 3.3↓ | Phosphoglycerate mutase, | |
| Glutamate metabolism | PF0201 | 3.4↓ | 2.4↓ | Aconitase |
| PF0202 | 3.7↓ | 2.1↓ | Isocitrate dehydrogenase | |
| PF0203 | 5.8↓ | 4.0↓ | Citrate synthase | |
| PF0204 | 2.8↓ | 3.6↓ | Glutamate synthase domain 1 | |
| PF0205 | 4.1↓ | 5.8↓ | Glutamate synthase domain 2 | |
| PF0206 | 2.9↓ | 3.2↓ | Glutamate synthase domain 3 | |
| PF0207 | 2.1↓ | 2.3↓ | Argininosuccinate synthase | |
| PF0450 | 35.9↓ | 25.2↓ | Glutamine synthetase | |
| PF1602 | 4.2 | NC | Glutamate dehydrogenase | |
| PF1852 | 1.9↓ | 6.6↓ | Glutamate synthase small subunit | |
| WOR5 | PF1480 | 5.2↓ | 4.7↓ | Aldehyde:ferredoxin oxidoreductase |
| Cofactor biosynthesis | PF1528 | 2.1↓ | 3.3↓ | Predicted glutamine amidotransferase |
| PF1529 | 3.5↓ | 5.9↓ | Pyridoxine biosynthesis enzyme | |
a
NC, no change (≤1.5-fold change); NA, not available; ↓, down-regulated.
b
HTH, helix-turn-helix; IOR, indolepyruvate ferredoxin oxidoreductase; KGOR, 2-ketoglutarate ferredoxin oxidoreductase; KOR, 2-ketoacid ferredoxin oxidoreductase.