TABLE 6.
Transcriptional responses of selected ORFs to S0 independent of glycoside type in P. furiosus
| Function | GeneID no. | Transcriptional response (fold change)a
|
Annotationb | |
|---|---|---|---|---|
| Cellobiose + S0 vs cellobiose | Maltose + S0 vs maltose | |||
| Purine metabolism | PF0153 | 4.1 | 2.1 | SAICAR synthase |
| PF0154 | 2.5 | 2.3 | Glutamine phosphoribosylpyrophosphate amidotransferase | |
| PF0426 | 4.3 | 4.0 | NCAIR synthetase | |
| PF1516 | 2.3 | 2.4 | GMP synthase, PP-ATPase domain/subunit | |
| PF1517 | 4.8 | 3.1 | Hypothetical protein | |
| Transposon | PF1366 | 4.1 | 2.0 | Transposase and inactivated derivatives |
| MBH membrane-bound hydrogenase | PF1433 | 12.2 | 7.1 | MbhK (NADH dehydrogenase subunit NuoC) |
| PF1434 | 11.0 | 6.7 | MbhL (Ni-Fe hydrogenase, NuoD) | |
| PF1435 | 11.6 | 7.2 | MbhM (NADH dehydrogenase subunit NuoH) | |
| MBX membrane-bound oxidoreductase | PF1441 | 9.7 | 7.7 | MbxN (four-cysteine proximal Fe-S binding protein) |
| PF1442 | 8.0 | 6.1 | MbxL (Ni-Fe hydrogenase, NuoD homolog) | |
| PF1443 | 8.7 | 6.1 | MbxK (NADH dehydrogenase subunit NuoC) | |
| PF1444 | 9.0 | 6.2 | MbxJ (four-cysteine proximal Fe-S binding protein) | |
| PF1445 | 9.9 | 7.9 | MbxM (NADH dehydrogenase subunit NuoH) | |
| PF1446 | 5.5 | 5.0 | MbxH′ (NADH dehydrogenase subunit NuoL) | |
| PF1448 | 7.3 | 4.7 | MbxG (membrane-bound hydrogenase, anchoring structure subunit) | |
| PF1450 | 3.3 | 2.5 | MbxD (membrane-bound hydrogenase, anchoring structure subunit) | |
| PF1451 | 7.6 | 5.4 | MbxC (membrane-bound hydrogenase, anchoring structure subunit) | |
| PF1452 | 6.0 | 4.1 | MbxB (membrane-bound hydrogenase, anchoring structure subunit) | |
| Sulfur-induced protein | PF2025 | 12.5 | 101 | SipA |
| PF2026 | 5.7 | 22.9 | SipB | |
a
NC, no change (≤1.5-fold change); NA, not available.
b
SAICAR, phosphoribosylaminoimidazole-succinocarboxamide synthase; NCAIR, phosphoribosylaminoimidazole carboxylase, ATPase subunit.