TABLE 2.
Gene targets, primer sequences, and PCR amplicon sizes in the multiplex PCR assay
| Gene target | Isolate group | Primer sequencea | Amplicon size (bp) | Protein encoded | Source or reference |
|---|---|---|---|---|---|
| LMOf2365_2798 | ECI | (F) AATAGAAATAAGCGGAAGTGT | 303 | Unknown function | This study |
| (R) TTATTTCCTGTCGGCTTAG | |||||
| LMOh7858_0487.8 to inlA | ECII | (F) ATTATGCCAAGTGGTTACGGA | 889 | Unknown function | This study |
| (R) ATCTGTTTGCGAGACCGTGTC | |||||
| LMOF6854_2463.4 | ECIII | (F) TTGCTAATTCTGATGCGTTGG | 497 | Putative helicase-like | This study |
| (R) GCGCTAGGGAATAGTAAAGG | |||||
| ORF2110 | Serotype 4b | (F) AGTGGACAATTGATTGGTGAA | 597 | Putative secreted | 5 |
| (R) CATCCATCCCTTACTTTGGAC | |||||
| lmo0737 | Serotype 1/2a | (F) GAGTAATTATGGCGCAACATC | 724 | Unknown function | This study |
| (R) CCAATCGCGTGAATATCGG | |||||
| lmo2234 | Listeria monocytogenes | (F) TGTCCAGTTCCATTTTTAACT | 420 | Unknown function | This study |
| (R) TTGTTGTTCTGCTGTACGA | |||||
| iap | Listeria spp. | (F) ATGAATATGAAAAAAGCAAC | 1,450-1,600 | P60 | 2 |
| (R) TTATACGCGACCGAAGCCAAC |
a
(F), forward primer; (R), reverse primer.