TABLE 3.
Catalytic propertiesa and other characteristics of EG1, rcCel9B, rcCel9BΔBTD, ClCBase, rcCel8B, and rcCel5H
| Enzyme (reference) | Km | Vmax (U/mg) | kcat (s−1) | kcat/Km (ml/s · mg) | Binding to cellulose | Temp optimum (oC) | pH optimum | Divalent cation requirement |
|---|---|---|---|---|---|---|---|---|
| EG1 (28) | 3.6 mg/ml | 84 | 91 | 25.3 | No | 39 | 6.5 | Yes |
| rcCel9B | 9.20 ± 0.83 mg/ml | 36.9 ± 1.46 | 39.79 ± 1.57 | 4.24 | No | 37 | 6.5 | Yes |
| rcCel9BΔBTD | 5.93 ± 0.78 mg/ml | 47.8 ± 3.00 | 46.29 ± 2.91 | 7.80 | No | 37 | 6.5 | Yes |
| rcCel5H | 16.2 ± 1.91 mg/ml | 0.31 ± 0.02 | 0.51 ± 0.03 | 0.031 | Yes | 37 | 6.5 | No |
| rcCel8B | 10.9 ± 1.25 mg/ml | 1.08 ± 0.06 | 1.47 ± 0.08 | 0.14 | No | 42 | 7.0 | No |
| ClCBase (14) | 0.1 mM | 14.2 | 15.9 | 159 | Yes | 45/37b | 6.5 | No |
| rcCel10A | 0.36 ± 0.02 mM | 11.6 ± 1.02 | 12.1 ± 1.06 | 33.6 | Yes | 37/37b | 6.5 | No |
a
Substrates used: p-nitrophenyl cellobioside for native ClCBase and rcCel10A and CMC for the enzymes EG1, rcCel9B, rcCel9BΔBTD, rcCel5H, and rcCel8B.
b
With/without 0.2 M NaCl.