FIG. 1.

Comparison of E. coli JM109 cells expressing AtPCS or SpPCS. (A) Cadmium accumulation in E. coli cells harboring either pQE60 (control), pQE-AtPCS (AtPCS), or pQE-SpPCS (SpPCS). The Cd content was measured by atomic absorption spectrometry. Data shown are the mean values (+ standard deviations) obtained from three independent experiments. (B) Western blot analysis of PCS expression in E. coli cells harboring either pQE60 (lane 2), pQE-AtPCS (lane 3), or pQE-SpPCS (lane 4). The molecular weight marker is shown in lane 1. The production of PCS was detected by an anti-His tag antibody, and the intensity was quantified using densitometry.