TABLE 2.
Nickel resistance screening of the libraries constructed with metagenomic DNA from the Rio Tinto rhizosphere
| Library | No. of clones | Avg insert size (kb) (range)a | Library size (Mb) | No. of Ni-resistant clonesb | No. of Ni-resistant clonesc/no. of unique Ni-resistant clonesd (plasmids) |
|---|---|---|---|---|---|
| A | 145,000 | 2 (1-5.5) | 290 | 884 | 10/5 (pSM1, pSM2, pSM3, pSM4 and pSM5) |
| B | 168,500 | 1.7 (1.2-3) | 286 | 630 | 18/2 (pSM11 and pSM12) |
| C | 102,000 | 2.5 (1-5) | 255 | 542 | 0/0 |
| D | 41,000 | 2 (1.3-3.3) | 82 | 351 | 6/4 (pSM6, pSM8, pSM9 and pSM10) |
| E | 270,000 | 4.5 (1-8.5) | 1,237 | 74 | 8/2 (pSM7 and pSM13) |
| Total | 726,500 | 2.5 (1-8.5) | 2,150 | 2,481 | 42/13 |
a
Estimated from restriction digestion analysis of 184 random clones using either EcoRI or EcoRI/XbaI enzymes.
b
Number of nickel resistance clones recovered after screening of the amplified library.
c
Number of nickel resistance clones recovered after retransformation.
d
Number of nickel resistance clones recovered after fragment length polymorphism analysis.