TABLE 2.
Primers selected for detection of N. ceranae and N. apis in multiplex PCR
| Primer | Sequencea | PCR product size (bp) | Specificity |
|---|---|---|---|
| 218MITOC-FOR | 5′-CGGCGACGATGTGATATGAAAATATTAA-3′ | 218-219b | N. ceranae |
| 218MITOC-REV | 5′-CCCGGTCATTCTCAAACAAAAAACCG-3′ | ||
| 321APIS-FOR | 5′-GGGGGCATGTCTTTGACGTACTATGTA-3′ | 321 | N. apis |
| 321APIS-REV | 5′-GGGGGGCGTTTAAAATGTGAAACAACTATG-3′ |
a
CG tails added to primers are underlined.
b
There is a 1-bp difference in the N. ceranae amplicon size depending on the sequences for N. ceranae available in GenBank (http://www.ncbi.nlm.nih.gov).