Skip to main content
. 2007 Aug 3;73(20):6331–6338. doi: 10.1128/AEM.00270-07

TABLE 3.

Sensitivity of the PCRs performed with the 218MITOC-FOR/218MITOC-REV and 321APIS-FOR/321APIS-REV primer pairs with spores diluted in water or uninfected honeybee maceratea

Primers Diluent No. positive/no. tested with the following no. of spores/100 μl (no. of spores/reaction mixture):
No. positive/no. tested with:
3 × 106 (15 × 104) 106 (5 × 104) 105 (5 × 103) 8 × 104 (4 × 103) 6 × 104 (3 × 103) 4 × 104 (2 × 103) 2 × 104 (103) 1.5 × 104 (750) 104 (500) 5 × 103 (250) 2 × 103 (100) 2 × 102 (10) 20 (1) Uninfected honeybee macerate Water
218 MITOC Water 6/6 6/6 6/6 6/6 6/6 6/6 5/6 5/6 3/6 0/6 0/6 0/6 0/6 0/6 0/6
Uninfected honeybee macerate 6/6 6/6 6/6 6/6 6/6 6/6 3/6 1/6 1/6 0/6 0/6 0/6 0/6 0/6 0/6
321APIS Water 6/6 6/6 6/6 6/6 6/6 2/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
Uninfected honeybee macerate 6/6 6/6 6/6 6/6 6/6 6/6 6/6 6/6 5/6 4/6 2/6 0/6 0/6 0/6 0/6
a

All samples were analyzed by three PCRs performed on three different days, and the amplicon products of each PCR were separated by electrophoresis twice.