General localization of the symbiont in nymphs and adults of C. columbae. Red and green signals indicate symbiont cells and host nuclei, respectively, while cuticles often exhibit red signals due to autofluorescence. Arrowheads, hybridization signals of bacteriocytes in the body cavity; arrows, hybridization signals associated with lateral oviducts, ovarial ampullae, and/or oocytes.