Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PCR products to confirm gene disruption. PCR was performed with sets of primers, namely, GADH1-4 and GADH1-5 (A and C) and SldA-1 and SldA-2 (B), and genomic DNA from the wild-type (lanes 1) and mutant (lanes 2) strains. PCR products were analyzed by agarose gel electrophoresis. (A) gndL::Km strain; (B) sldA::Km strain; (C) gndL::Tc sldA::Km strain. Lanes M, λ DNA/StyI marker DNA.
