TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant trait(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF) recA1 (rK− mK+) | TOYOBO |
| “G. dioxyacetonicus” IFO 3271 (G. frateurii NBRC 3271) strains | ||
| Wild type | IFO or NBRC | |
| gndL::Km mutant | Kmr, gndL disruptant | This study |
| sldA::Km mutant | Kmr, sldA disruptant | This study |
| gndL::Tc mutant | Tcr, gndL disruptant | This study |
| gndL::Tc sldA::Km mutant | Tcr Kmr, gndL and sldA double disruptant | This study |
| Plasmids | ||
| pUC4K | Contains nonpolar Kmr cassette | Pharmacia |
| pKRP12 | Contains Tcr cassette | 16 |
| pSUP202sldA::Km | Kmr, mutation for sldA disruption | 10 |
| pUC119-BP | 4.3-kb BamHI-PstI fragment in pUC119 | This study |
| pUC119-BPgndL::Km | Kmr, for gndL disruption; Kmr cassette inserted at SalI site | This study |
| pUC119-BPgndL::Tc | Tcr, for gndL disruption; Tcr cassette inserted at SalI site | This study |