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(A) M. maripaludis colonies expressing M. kandleri nrpR (strain Mm1058) or His-tagged M. maripaludis nrpR (strain Mm1048). Colonies were grown with ammonia or N2 as a nitrogen source. Numbers below images are β-galactosidase activities (Miller units) from the same strains grown in liquid medium with the same nitrogen sources. (B) Screen for random mutants of M. kandleri NrpR. Lighter-colored mutant colonies are indicated by arrows.
