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. 2007 Aug 24;73(20):6595–6600. doi: 10.1128/AEM.01324-07

FIG. 5.

FIG. 5.

(A) nif regulation by mutants of M. kandleri NrpR resulting from random and site-specific mutagenesis. Vector and wild-type controls are as described in the legend of Fig. 4. Mm1119, Mm1120, and Mm1124 are mutant strains isolated after random mutagenesis. C167A and S168A are M. kandleri NrpR site-specific mutants. Black bars, ammonia-grown cultures; white bars, dinitrogen-grown cultures. (B) nif regulation by wild-type M. maripaludis NrpR and site-specific mutants. Vector control, strain Mm1171 (no NrpR); wild type, strain Mm1048 (His-tagged M. maripaludis NrpR); black bars, ammonia-grown cultures; white bars, dinitrogen-grown cultures.