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Assessment of rDNA copy numbers in in vitro-induced P. piscicida cysts. DNA was extracted from P. piscicida cysts collected from cultures of encysted cells without (A) and with (B) the presence of autoclaved sediment. The assessment of rDNA copy numbers was performed by species-specific QC-PCR for P. piscicida (62). rfu, relative fluorescence units.
