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. 2007 Aug 17;73(20):6410–6420. doi: 10.1128/AEM.01229-07

FIG. 6.

FIG. 6.

Detection of P. piscicida in sediments collected from fish farm ponds. DNA was extracted from 0.1 g (wet weight) of sediment boiled with TE (A), 0.1 g (wet weight) of sediment subjected to extraction with the BIO101 FastDNA spin kit for soil (B), the supernatant (15 ml) of a suspension (50 ml) of sediment (10 ml) in ASW (C), the supernatant of a suspension (the same quantities as for panel C) in f/2 medium after incubation at 23°C for 1 week (D), and the supernatant of a suspension (the same quantities as for panel C) in ASW with an adult fish after incubation at 23°C for 1 week (E). Sample numbers correspond to the sediments collected from different fish farming ponds (1 to 10). Positive and negative controls are indicated.