TABLE 4.

Counts (log₁₀) of total bacteria per ml (using the universal probe Eub338) and several bacterial groups (using the probes Bac303, Erec482, Bif164, Prop853, Ehal1469, and Acac194) at 0 (initial) and 24 h of incubation in batch cultures containing a mixture of carbohydrates, lactate, or both and inoculated with fecal slurries from four different volunteers

Substrate and condition	Count (log10) of total bacteria per ml
	Eub	Bac	Erec	Bif	Prop	Ehal	Acac
Initial counta	7.93	7.29	7.43	6.74	7.19	4.88	4.18
SDb	0.095	0.111	0.091	0.243	0.207	0.464	0.429
Substrate
Mixture
pH 5.2	8.24	6.60	7.27	7.96	6.58	5.53	5.06
pH 5.9	8.29	7.09	7.50	7.83	7.02	6.05	5.21
pH 6.4	8.62	7.58	7.76	7.76	7.59	6.79	5.39
Mixture plus lactate
pH 5.2	8.26	6.84	7.02	7.97	6.49	5.42	4.88
pH 5.9	8.44	7.16	7.70	7.94	7.17	7.23	5.34
pH 6.4	8.69	7.26	7.99	7.93	7.52	7.25	5.29
Lactate
pH 5.2	7.93	6.88	7.00	6.83	6.82	5.95	4.69
pH 5.9	8.02	6.92	7.62	6.47	6.83	6.06	5.22
pH 6.4	8.30	7.18	7.48	6.67	7.02	6.73	5.48
SEDc	0.098	0.257	0.144	0.169	0.220	0.197	0.141

	P valued
	Eub	Bac	Erec	Bif	Prop	Ehal	Acac
pH	<0.001	0.003	<0.001	0.20	<0.001	<0.001	<0.001
Substrate	<0.001	0.76	0.006	<0.001	0.32	<0.001	0.56
pH × substrate	0.92	0.38	0.031	0.65	0.06	<0.001	0.10

^aEstimated from fecal counts and taking into account the slurry preparation.

^bSD refers to the standard deviation in counts across volunteers.

^cSED, standard error of the difference.

^dAnalyzed as two-way ANOVA with volunteer as random effect and substrate, pH, and their interaction as fixed effects.