TABLE 4.

Quantitative amplification parameters for the Q-PCR assays in leachate and SBD

Q-PCR assay	Sample	Target	Amplification parametera				Linear range (no. of cells or spores)
			E	Slope	y intercept	R2
ITS	Leachate	B. atrophaeus vegetative cells	0.99 ± 0.07	−3.34 ± 0.18	37.7 ± 0.8	>0.98	101-107
		B. atrophaeus spores	0.97 ± 0.04	−3.39 ± 0.12	38.7 ± 0.6	>0.98	101-107
	SBD	B. atrophaeus vegetative cells	0.96 ± 0.05	−3.42 ± 0.13	36.7 ± 0.6	>0.98	101-107
		B. atrophaeus spores	1.04 ± 0.22	−3.24 ± 0.52	39.6 ± 2.7	>0.98	101-107
	Sterile water	B. atrophaeus vegetative cells	0.93 ± 0.08	−3.51 ± 0.21	38.6 ± 1.0	>0.98	101-107
recABa	SBD	B. atrophaeus spores	0.87 ± 0.01	−3.66 ± 0.04	39.9 ± 0.3	>0.99	102-107
gyrB	Leachate	S. marcescens	1.04 ± 0.04	−3.24 ± 0.09	39.4 ± 0.6	>0.98	102-107
	SBD	S. marcescens	0.82 ± 0.07	−3.85 ± 0.28	43.9 ± 1.4	>0.99	102-107
recASm	Leachate	S. marcescens	1.03 ± 0.05	−3.26 ± 0.11	43.1 ± 0.7	>0.97	102-107
	SBD	S. marcescens	0.78 ± 0.03	−3.98 ± 0.11	46.7 ± 0.4	>0.99	102-107
wzm	Leachate	S. marcescens	0.94 ± 0.05	−3.47 ± 0.13	38.9 ± 0.5	>0.98	102-107
	SBD	S. marcescens	0.82 ± 0.02	−3.86 ± 0.07	40.6 ± 0.4	>0.99	102-107
	Sterile water	S. marcescens	0.81 ± 0.03	−3.87 ± 0.12	41.2 ± 0.9	>0.99	102-107

^aThe value for each parameter except R² is a mean ± standard deviation of two DNA extractions (corresponding to two spiking experiments), each quantified in triplicate on the same plate (n = 6).