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Kinetics of growth-related parameters of the secretion-engineered P. pastoris and the control strain in fed-batch cultures. Development of biomass concentration (g liter−1 yeast dry mass [YDM]) (A) and specific growth rates (μ) (B) are shown for the P. pastoris expression strains (SMD1168 secreting 2F5 Fab under the control of the GAP promoter) coexpressing the novel secretion helper factors and the control strain expressing only the antibody fragment.
