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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2007 May 10;223(1):1–9. doi: 10.1016/j.taap.2007.04.014

Figure 7.

Figure 7

Intracellular oxidative stress in cells collected via BAL from IFN-γ-primed and particle-exposed mice. Mice were exposed to IFN-γ (I) or PBS (P) aerosol for 15 min., then to intranasal PBS (P) or CAPs (C) 3 hours later. BAL was performed 24 hours later. The collected cells were incubated for 30 min. at 37°C with DCFH and anti-Gr-1 antibody, which binds specifically to PMNs. Intracellular oxidative stress was assessed via flow cytometry, with green fluorescence indicative of intracellular oxidation of DCFH. The cells were gated by their red fluorescence (anti-Gr-1 antibody). Top panel: AMs. Bottom panel: PMNs. Data represent the mean ± SEM of at least 3 independent experiments. * = p<0.05 compared to PP group.