Figure 3. Effect of DEDTC on intrinsic cellular properties.

CA3 pyramidal cells were recorded in whole-cell current-clamp at membrane potential below the level of spontaneous firing. A and B, superimposed traces represent response to ramp-(A) and step-depolarization (B) in control (black) and after application of 200 μm DEDTC (red) or 1 μm TPEN (light blue). C, graphs indicate that intra- and extracellular chelation with DEDTC or TPEN has no effect on the interspike interval, but tends to change the homogeneity of spike timing in individual cells.