Skip to main content
. 2006 Oct 12;578(Pt 1):131–142. doi: 10.1113/jphysiol.2006.114959

Figure 3. Replacing the N-terminal domain of NBCe1-A with the N-terminal domain of NBCn1-B.

Figure 3

A, chimeric transporter n1(Nt)/e1. B, (I–V) relationships obtained from oocytes expressing n1(Nt)/e1 (n = 7) in the absence (○) and presence (•) of 5% CO2–26 mm HCO3− (pH 7.4). The difference IHCO3 – IND96 is an estimate of the electrogenic cotransport current (▴). C, representative recordings of pHi and membrane potential (Vm) in an oocyte expressing n1(Nt)/e1. The pHi recovery from a CO2-induced acidification (arrow) and the hyperpolarization upon application of 1.5%CO2–10 mm HCO3− (arrowhead) are hallmarks of electrogenic Na+–HCO3− transport. One of four experiments.