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. 2007 Mar 8;581(Pt 2):553–565. doi: 10.1113/jphysiol.2006.126417

Figure 3. Mechanisms of adenosine release.

Figure 3

A, superimposed current traces from an adenosine biosensor placed on the surface of the molecular layer in control, in 10 μm CNQX + 50 μm AP5 and in 0.5 μm TTX. The adenosine release following electrical stimulation (5 V, 8 s at arrow) was abolished by TTX but was insensitive to the block of glutamate receptors. B, superimposed traces from an adenosine biosensor placed on the surface of the molecular layer in control, Ca2+-free aCSF (3.7 mm Mg2+) and following reintroduction of Ca2+ (wash). The adenosine release following electrical stimulation (7 V, 8 s at arrow) was abolished by removal of Ca2+. Inset, graph plotting normalized adenosine release against external Ca2+ concentration. Adenosine release was normalized to what occurred at 2.4 mm Ca2+ and summarizes data from 6 slices. C, superimposed traces from an adenosine biosensor placed on the surface of the molecular layer in control and in the presence of the GABAA receptor agonist muscimol (30 μm). Adenosine was released by a 5 V, 7 s stimulus at the arrow. Inset, superimposed averages of EPSPs in control and in 30 μm muscimol (*). Muscimol reduced EPSP amplitude but had little effect on the volley. D, superimposed traces from an adenosine biosensor placed on the surface of the molecular layer in control and in the presence of 5 μm NBTI and 10 μm dipyridamole to block equilibrative transport. Adenosine was released by a 5 V, 7 s stimulus at the arrow. All stimuli were at a frequency of 20 Hz.