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. 2007 Mar 8;581(Pt 2):553–565. doi: 10.1113/jphysiol.2006.126417

Figure 5. Metabolism of ɛ-ATP by cerebellar slices at room temperature.

Figure 5

A, graph plotting percentage composition against time for the breakdown of 50 μmɛ-ATP. At time zero there was ∼90% ATP (▪) and 10% ADP (▴). Following incubation there was a fall in the proportion of ATP and a subsequent increase in AMP (□) and adenosine (^) with little change in ADP. B, graph plotting percentage of 50 μmɛ-ATP remaining against time in control and in the presence of inhibitors (Evans Blue and ARL67156). C, graph plotting percentage of adenosine against time in control and in the presence of inhibitors during metabolism of 50 μmɛ-ATP. α,β-Methylene-ADP (100 μm) was the most effective in slowing the build-up of adenosine. D, graph plotting percentage composition against time for the breakdown of 5 μmɛ-ATP. There was a more rapid breakdown of 5 μm ATP compared with 50 μmɛ-ATP (compare with A). E, graph plotting percentage of ɛ-ATP (5 μm) remaining against time in control and in the presence of inhibitors. Note that both Evans Blue and ARL67156 (100 μm) were more effective at slowing the metabolism of 5 μmversus 50 μmɛ-ATP (compare E and B). F, graph plotting percentage of adenosine against time in control and in the presence of inhibitors during metabolism of 5 μmɛ-ATP. Again inhibitors are more effective at slowing build up of adenosine with lower concentrations of substrate. Graphs A, B and C summarize data from 4 experiments; graphs D, E and F are from 3 experiments.