Figure 4. Neurally evoked contractions of control and hpgT vasa deferentia were reduced by either prazosin or suramin but those of hpg vasa deferentia were only reduced by prazosin.

A, overlaid traces showing contractions of the circular smooth muscle of control (upper traces), hpg (middle traces) and hpgT (lower traces) vasa deferentia evoked by 20 stimuli at 10 Hz under control conditions (thin line) and in the presence of either prazosin (0.1 μm) or suramin (0.1 mm) (thick line). B and C, bar graphs showing the relative change in contraction of control, hpg and hpgT vasa deferentia produced by prazosin (B) and suramin (C). Comparisons with control recordings in the same tissues were made with paired t tests (**P < 0.01, ***P < 0.001). Comparisons between the groups of tissues were made with one-way ANOVA followed by Games–Howell tests (†P < 0.05, †††P < 0.001).