Figure 3.

Whole cell fucosidase activity on PNPF of the pool of colonies selected after each round of DNA shuffling. Rounds 1–7 are pools of colonies. Also shown are the activity of cells expressing the native β-galactosidase and cells expressing the evolved β-fucosidase, both measured as whole cell activity of single clones. The evolved fucosidase is the single-best colony selected after quantitative comparison of the 24 best colonies from the pool of colonies obtained after shuffling round 7. For assay conditions, see Materials and Methods.