Table 1.
Summary of the effects of temperature, menthol and BCTC on TRPM8 activation in HEK293 cells
| Control 33°C | Cold 20°C | 100 μm menthol 33°C | Cold + 100 μm menthol | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| no drug | 3 BCTC | no drug | 1 BCTC | 3 BCTC | no drug | 1 BCTC | 3 BCTC | no drug | 1 BCTC | 3 BCTC | |
| s (mV−1) | 36 ± 2 | 54 ± 8 | 35 ± 1 | 40 ± 3 | 45 ± 4* | 38 ± 2 | 33 ± 3 | 36 ± 1 | 40 ± 2 | 48 ± 5 | 41 ± 1 |
| g (nS) | 38 ± 3 | 25 ± 11 | 52 ± 5 | 48 ± 14 | 19 ± 6‡; | 78 ± 7 | 60 ± 10 | 43 ± 7†; | 86 ± 7 | 64 ± 19 | 56 ± 5†; |
| V1/2 (mV) | 215 ± 4 | 229 ± 11 | 153 ± 5 | 193 ± 19* | 223 ± 10‡; | 89 ± 6 | 112 ± 16 | 172 ± 7‡; | 58 ± 4 | 122 ± 33 | 153 ± 9‡; |
| n | 39 | 6 | 26 | 3 | 8 | 29 | 8 | 21 | 24 | 3 | 15 |
The absolute values of the parameters s, g and V1/2 obtained from the fits of the ramp (−100 to +200 mV) I–V curves to eqn (6) in the Methods. The data are given as means ±s.e.m. and are grouped according to the different experimental conditions (control or agonist) onto which BCTC was applied. To assess statistical significance, unpaired t test was performed between the parameters obtained in the absence (‘no drug’) and presence of BCTC for each antagonist condition, and significance indicated:
*
P < 0.05
†
P < 0.001
‡
P < 0.001.