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. 2007 Mar 8;582(Pt 3):977–990. doi: 10.1113/jphysiol.2007.128413

Figure 1. Effects of membrane stretch on LKbg channel activity in inside-out (i-o) and cell-attached (c-a) patches.

Figure 1

A, for i-o patches, negative pressure was applied through a pipette after confirming inhibition of LKbg channels by MgATP (1 mm). Throughout the figures, the letter ‘c’ and horizontal arrows indicate the closed and open levels of LKbg channels, respectively. B, summary of normalized open probability (Po) measured at various negative pressures and different MgATP concentrations. C, time course of the increase in Po induced by membrane stretch. Mean values of normalized Po (100 ×Po/Po,max (%); Po,max was obtained under MgATP-free conditions) were plotted every 10 s under continuous negative pipette pressure; −13 (n = 11, ▪), −20 (n = 12, ○) and −27 mmHg (n = 13, ▴). D and E, a representative trace of c-a recording and summary of Po responses to negative pressure (−27 mmHg).