Figure 4. Effects of MβCD and membrane stretch on recovery from the PIP₂-induced inhibition of LK_bg channels.

A, by a sustained application of PIP₂ (e.g. > 1 min after confirming the inhibition of channels), the LK_bg channel activity was not recovered by washout of PIP₂ up to 20 min. B, an immediate and prolonged washout of PIP₂ (< 20 s after confirming the inhibition) induced a partial recovery of LK_bg channel activity. The summarized results are shown as a bar graph (n = 9, right panel). C, recovery of LK_bg channels from inhibition by PIP₂ was facilitated by adding MβCD (2 mm). Right panel, summary of the mean P_o (n = 6). D, membrane stretch (−20 or −27 mmHg) combined with MβCD also facilitated LK_bg channel recovery from the previous application of 2 μm PIP₂ (dotted line, PIP₂ washout). Similar results were obtained in three more cases. All the above experiments were i-o recordings.