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. 2007 Apr 26;582(Pt 1):73–85. doi: 10.1113/jphysiol.2007.128843

Figure 5. Effect of thiamine deficiency on hTHTR-1 and hTHTR-2 mRNA and protein levels and on activity of SLC19A2 and SLC19A3 promoters in ARPE-19 cells.

Figure 5

Real-time PCR analysis of hTHTR-1 (A) and hTHTR-2 (B) mRNA levels in ARPE-19 cells grown under control (12 μm) and thiamine deficient (1 nm) conditions. Data are from at least three independent sets of experiments and normalized against β-actin and then quantified as described in the Methods. Western blot analysis of hTHTR-1 (C) (relative density of the bands were determined and are as follows: for hTHTR-1 the intensity was 946385 and 1100047 and for β-actin the intensity was 707596 and 32997 for control and thiamine-deficient cells, respectively) and hTHTR-2 (D) proteins in membranous fractions of ARPE-19 cells grown under control (12 μm) and thiamine-deficient (1 nm) conditions. Data shown are representative of three separate sets of experiments. Activity of full-length SLC19A2 (E) and SLC19A3 (F) promoters in ARPE-19 cells grown in control (12 μm) and thiamine-deficient (1 nm) conditions. Data are means ±s.e.m. of three separate determinations performed on different occasions.