Table 1.

Gene-specific primers used for generating mutations in SLC19A2

Amino acid	Forward and reverse primers 5′–3′
hTHTR-1	CCGCTCGAGATGGATGTGCCCGGCC
	CGGGATCCTGAAGTGGTTACTTGAGAACT
D93H	GTTTCCTGTGTTCCTTGCCACACACTACCTCCGTTATAAAC
	GTTTATAACGGAGGTAGTGTGTGGCAAGGAACACAGGAAAC
S143F	ATTGCCTATTACTTTTATATCTACAGTGTGGTGGACCTGGGC
	GCCCAGGTCCACCACACTGTAGATATAAAAGTAATAGGCAAT
G172D	GGTGGGCTTTACAGTGGACTCTGTCCTAGGGCAAATCC
	GGATTTGCCCTAGGACAGAGTCCACTGTAAAGCCCACC

The table shows the required nucleotide changes in forward and reverse primers (bold face) used for generation of point mutations. Restriction sites for XhoI (italics) and BamHI (underlined) were incorporate into the SLC19A2 primer to aid subcloning into the GFP–N3 vector.